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Santa Cruz Biotechnology slug
Slug, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 575 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/slug/SLUG+Antibody/10__1096_slash_fj__202504700r-101-57-58
Average 96 stars, based on 575 article reviews
slug - by Bioz Stars, 2026-10
96/100 stars

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other:

Article Title: Caffeic Acid Derivative MPMCA Inhibits Prostate Cancer EMT and Metastasis by Regulating Transcription Factors Snail and Slug
Article Snippet: The β-actin (SC-47778), β-Catenin (SC-133240), Vimentin (SC-6260), ZO-1 (SC-33725), Snail (SC-271977), Slug (SC-166476), p-PI3-kinase p85a (SC-12929), PI3-kinase p85a (SC-1637), AKT (SC-5298), PI3K activator (SC-3036), and AKT activator (Fumonisin B1; SC-201395) were obtained from Santa Cruz Biotechnology (Santa Cruz Biotechnology, Dallas, TX, USA). p-mTOR (5536S), mTOR (2983S), N-cadherin (ab76057), p-AKT (4060S), and mTOR activator (MHY1485; CAS Number: 17949) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Article Title: Caffeic Acid Derivative MPMCA Inhibits Prostate Cancer EMT and Metastasis by Regulating Transcription Factors Snail and Slug
Article Snippet: The β-actin https://doi.org/10.3390/cells15050454 (SC-47778), β-Catenin (SC-133240), Vimentin (SC-6260), ZO-1 (SC-33725), Snail (SC-271977), Slug (SC-166476), p-PI3-kinase p85a (SC-12929), PI3-kinase p85a (SC-1637), AKT (SC-5298), PI3K activator (SC-3036), and AKT activator (Fumonisin B1; SC-201395) were obtained from Santa Cruz Biotechnology (Santa Cruz Biotechnology, Dallas, TX, USA). p-mTOR (5536S), mTOR (2983S), N-cadherin (ab76057), p-AKT (4060S), and mTOR activator (MHY1485; CAS Number: 17949) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Article Title: Analysis of Periostin, TGF-β, and SLUG Expression in Inflammatory Bowel Disease in Pediatric Patients and Their Clinical Implications.
Article Snippet: IHC were performed on 4 μm thick paraffin sections using Autostainer Link48 (Agilent, Santa Clara, CA, USA).

Blocking Assay:

Article Title: ANKRD49 promotes the epithelial-mesenchymal transition of non-small cell lung cancer via the PKNOX1/TGF-β1/SMAD axis
Article Snippet: .. After blocking with 5% skimmed milk or 5% BSA, the membranes were incubated with indicated primary antibodies at 4 °C overnight, and then incubated with anti-mouse or anti-rabbit HRP-labeled secondary antibodies at room temperature for 1 h. The primary antibodies used in this study are as following: ANKRD49 (Cat# 25034-1-AP, 1:1000), PKNOX1 (Cat# 10614-1-AP, 1:750), Twist (Cat# 25465-1-AP, 1:1000, Protein Tech, USA), E-cadherin (Cat# 3195 S, 1:1000), EpCAM (Cat# 93790, 1:1000), N-cadherin (Cat# 13116, 1:1000), ZEB1(Cat# 70512, 1:1000), Vimentin (Cat# 5741 S, 1:1000, Cell Signaling Technology, USA), Slug (Cat# sc-166476, 1:1000), Snail(Cat# sc-2719777, 1:1000), α-SMA (Cat# sc-53142, 1:1000, Santa Cruz Biotechnology, USA), TGF-β1 (Cat# BA0290, 1:1000, Boster Biological Technology, China), SMAD2 (Cat# D155233, 1:1000), SMAD3 (Cat# D161451, 1:1000, Sangon Biotech, China), P-SMAD2 (Cat# AP0269, 1:1000, ABclonal, China) and P-SMAD3 (Cat# AF1759, 1:1000, Beyotime Biotechnology, China), Tubulin monoclonal antibody (Cat# CPA9126, 1:5000, Cohesion Biosciences, UK) or GAPDH monoclonal antibody (Cat# bsm-33033 M, 1:5000, Bioss Biotechnology, China). .. Finally, the membranes were incubated with enhanced ECL detection reagent (Cat# MA0186, Meilunbio, China) and imaged using AlphaImager MINI system (ProteinSimple, USA).

Article Title: Modulation of β‐Catenin/ EMT Axis by Croton caudatus and Its Phytoflavonoid Kaempferol Ameliorates Cervical Cancer Migration and Stemness
Article Snippet: Proteins were loaded at 100 μg/lane, separated by 8% (β- catenin, E- cadherin, N- cadherin, Zeb2, Importin β- 1, Phospho- β- catenin, MMP- 9), 10% (Vimentin, OCT4, SOX2, αTUBULIN, GSK3β, c- MYC, Cyclin D1), and 12% (Twist, Slug, 14- 3- 3, MMP- 7, Histone H1) SDS- PAGE and transferred to PVDF membrane. .. Membranes were incubated with primary antibodies overnight against β- catenin (Affinity Biosciences Cat# AF6266, RRID:AB_2835124), E- cadherin (Santa Cruz Biotechnology Cat# sc- 7870, RRID:AB_2076666), N- cadherin (Thermo Fisher Scientific Cat# PA5- 87437, RRID:AB_2804146), Zeb2 (Thermo Fisher Scientific Cat# PA5- 95090, RRID:AB_2806896), Vimentin (Thermo Fisher Scientific Cat# PA5- 142829, RRID:AB_2933472), Snail (Santa Cruz Biotechnology Cat# sc- 271977, RRID:AB_10709902), Slug (Santa Cruz Biotechnology Cat# sc166476, RRID:AB_2191897), Twist (Santa Cruz Biotechnology Cat# sc- 15393, RRID:AB_2211738), Importin β- 1 (Cell signaling Technology, Cat# 60769T), 14- 3- 3 (Santa Cruz Biotechnology, Cat# sc732), GSK3β (Elabscience, Cat# E- AB- 22221), Phosphoβ- catenin (Elabscience Cat# E- AB- 20228), c- MYC (Santa Cruz Biotechnology, Cat# sc- 40, RRID:AB_627268), Cyclin D1 (Santa Cruz Biotechnology, Cat# sc8396, RRID:AB_627344), MMP- 7 (Elabscience Cat# E- AB- 10062), MMP- 9 (Abcam Cat# ab38898, RRID:AB_776512), OCT4 (Santa Cruz Biotechnology Cat# sc5279, RRID:AB_628051), SOX2 (Santa Cruz Biotechnology Cat# sc- 365964, RRID:AB_10843364), α- TUBULIN (Thermo Fisher Scientific, USA, Cat# PA5- 85922, RRID:AB_2802723), Histone H1 (Thermo Fisher Scientific, USA, Cat# PA5- 30055, RRID:AB_2547529), after blocking with 5% nonfat dried milk, followed by incubation with HRP- Goat Anti- Rabbit (Thermo Fisher Scientific, USA, Cat# 31460, RRID:AB_228341)/HRPGoat Anti- Mouse secondary antibodies (Thermo Fisher Scientific, USA, Cat# 31430, RRID:AB_228307), AP- Rabbit Anti- Mouse IgG + A + M [H + L] (Thermo Fischer scientific, USA Cat #31329, RRID:AB_228424), AP- Goat Anti- Rabbit IgM (Thermo Fischer scientific, USA, Cat # G- 21079, RRID: AB_2536529), for 1 h at room temperature [27]. .. Proteins were detected using ECL method as well as alkaline phosphatase method; densitometric scanning of protein bands were performed in Bio- Rad Gel Doc XR+ system and analyzed using Image Lab software.

Incubation:

Article Title: ANKRD49 promotes the epithelial-mesenchymal transition of non-small cell lung cancer via the PKNOX1/TGF-β1/SMAD axis
Article Snippet: .. After blocking with 5% skimmed milk or 5% BSA, the membranes were incubated with indicated primary antibodies at 4 °C overnight, and then incubated with anti-mouse or anti-rabbit HRP-labeled secondary antibodies at room temperature for 1 h. The primary antibodies used in this study are as following: ANKRD49 (Cat# 25034-1-AP, 1:1000), PKNOX1 (Cat# 10614-1-AP, 1:750), Twist (Cat# 25465-1-AP, 1:1000, Protein Tech, USA), E-cadherin (Cat# 3195 S, 1:1000), EpCAM (Cat# 93790, 1:1000), N-cadherin (Cat# 13116, 1:1000), ZEB1(Cat# 70512, 1:1000), Vimentin (Cat# 5741 S, 1:1000, Cell Signaling Technology, USA), Slug (Cat# sc-166476, 1:1000), Snail(Cat# sc-2719777, 1:1000), α-SMA (Cat# sc-53142, 1:1000, Santa Cruz Biotechnology, USA), TGF-β1 (Cat# BA0290, 1:1000, Boster Biological Technology, China), SMAD2 (Cat# D155233, 1:1000), SMAD3 (Cat# D161451, 1:1000, Sangon Biotech, China), P-SMAD2 (Cat# AP0269, 1:1000, ABclonal, China) and P-SMAD3 (Cat# AF1759, 1:1000, Beyotime Biotechnology, China), Tubulin monoclonal antibody (Cat# CPA9126, 1:5000, Cohesion Biosciences, UK) or GAPDH monoclonal antibody (Cat# bsm-33033 M, 1:5000, Bioss Biotechnology, China). .. Finally, the membranes were incubated with enhanced ECL detection reagent (Cat# MA0186, Meilunbio, China) and imaged using AlphaImager MINI system (ProteinSimple, USA).

Article Title: Modulation of β‐Catenin/ EMT Axis by Croton caudatus and Its Phytoflavonoid Kaempferol Ameliorates Cervical Cancer Migration and Stemness
Article Snippet: Proteins were loaded at 100 μg/lane, separated by 8% (β- catenin, E- cadherin, N- cadherin, Zeb2, Importin β- 1, Phospho- β- catenin, MMP- 9), 10% (Vimentin, OCT4, SOX2, αTUBULIN, GSK3β, c- MYC, Cyclin D1), and 12% (Twist, Slug, 14- 3- 3, MMP- 7, Histone H1) SDS- PAGE and transferred to PVDF membrane. .. Membranes were incubated with primary antibodies overnight against β- catenin (Affinity Biosciences Cat# AF6266, RRID:AB_2835124), E- cadherin (Santa Cruz Biotechnology Cat# sc- 7870, RRID:AB_2076666), N- cadherin (Thermo Fisher Scientific Cat# PA5- 87437, RRID:AB_2804146), Zeb2 (Thermo Fisher Scientific Cat# PA5- 95090, RRID:AB_2806896), Vimentin (Thermo Fisher Scientific Cat# PA5- 142829, RRID:AB_2933472), Snail (Santa Cruz Biotechnology Cat# sc- 271977, RRID:AB_10709902), Slug (Santa Cruz Biotechnology Cat# sc166476, RRID:AB_2191897), Twist (Santa Cruz Biotechnology Cat# sc- 15393, RRID:AB_2211738), Importin β- 1 (Cell signaling Technology, Cat# 60769T), 14- 3- 3 (Santa Cruz Biotechnology, Cat# sc732), GSK3β (Elabscience, Cat# E- AB- 22221), Phosphoβ- catenin (Elabscience Cat# E- AB- 20228), c- MYC (Santa Cruz Biotechnology, Cat# sc- 40, RRID:AB_627268), Cyclin D1 (Santa Cruz Biotechnology, Cat# sc8396, RRID:AB_627344), MMP- 7 (Elabscience Cat# E- AB- 10062), MMP- 9 (Abcam Cat# ab38898, RRID:AB_776512), OCT4 (Santa Cruz Biotechnology Cat# sc5279, RRID:AB_628051), SOX2 (Santa Cruz Biotechnology Cat# sc- 365964, RRID:AB_10843364), α- TUBULIN (Thermo Fisher Scientific, USA, Cat# PA5- 85922, RRID:AB_2802723), Histone H1 (Thermo Fisher Scientific, USA, Cat# PA5- 30055, RRID:AB_2547529), after blocking with 5% nonfat dried milk, followed by incubation with HRP- Goat Anti- Rabbit (Thermo Fisher Scientific, USA, Cat# 31460, RRID:AB_228341)/HRPGoat Anti- Mouse secondary antibodies (Thermo Fisher Scientific, USA, Cat# 31430, RRID:AB_228307), AP- Rabbit Anti- Mouse IgG + A + M [H + L] (Thermo Fischer scientific, USA Cat #31329, RRID:AB_228424), AP- Goat Anti- Rabbit IgM (Thermo Fischer scientific, USA, Cat # G- 21079, RRID: AB_2536529), for 1 h at room temperature [27]. .. Proteins were detected using ECL method as well as alkaline phosphatase method; densitometric scanning of protein bands were performed in Bio- Rad Gel Doc XR+ system and analyzed using Image Lab software.

Cell-Signaling:

Article Title: Modulation of β‐Catenin/ EMT Axis by Croton caudatus and Its Phytoflavonoid Kaempferol Ameliorates Cervical Cancer Migration and Stemness
Article Snippet: Proteins were loaded at 100 μg/lane, separated by 8% (β- catenin, E- cadherin, N- cadherin, Zeb2, Importin β- 1, Phospho- β- catenin, MMP- 9), 10% (Vimentin, OCT4, SOX2, αTUBULIN, GSK3β, c- MYC, Cyclin D1), and 12% (Twist, Slug, 14- 3- 3, MMP- 7, Histone H1) SDS- PAGE and transferred to PVDF membrane. .. Membranes were incubated with primary antibodies overnight against β- catenin (Affinity Biosciences Cat# AF6266, RRID:AB_2835124), E- cadherin (Santa Cruz Biotechnology Cat# sc- 7870, RRID:AB_2076666), N- cadherin (Thermo Fisher Scientific Cat# PA5- 87437, RRID:AB_2804146), Zeb2 (Thermo Fisher Scientific Cat# PA5- 95090, RRID:AB_2806896), Vimentin (Thermo Fisher Scientific Cat# PA5- 142829, RRID:AB_2933472), Snail (Santa Cruz Biotechnology Cat# sc- 271977, RRID:AB_10709902), Slug (Santa Cruz Biotechnology Cat# sc166476, RRID:AB_2191897), Twist (Santa Cruz Biotechnology Cat# sc- 15393, RRID:AB_2211738), Importin β- 1 (Cell signaling Technology, Cat# 60769T), 14- 3- 3 (Santa Cruz Biotechnology, Cat# sc732), GSK3β (Elabscience, Cat# E- AB- 22221), Phosphoβ- catenin (Elabscience Cat# E- AB- 20228), c- MYC (Santa Cruz Biotechnology, Cat# sc- 40, RRID:AB_627268), Cyclin D1 (Santa Cruz Biotechnology, Cat# sc8396, RRID:AB_627344), MMP- 7 (Elabscience Cat# E- AB- 10062), MMP- 9 (Abcam Cat# ab38898, RRID:AB_776512), OCT4 (Santa Cruz Biotechnology Cat# sc5279, RRID:AB_628051), SOX2 (Santa Cruz Biotechnology Cat# sc- 365964, RRID:AB_10843364), α- TUBULIN (Thermo Fisher Scientific, USA, Cat# PA5- 85922, RRID:AB_2802723), Histone H1 (Thermo Fisher Scientific, USA, Cat# PA5- 30055, RRID:AB_2547529), after blocking with 5% nonfat dried milk, followed by incubation with HRP- Goat Anti- Rabbit (Thermo Fisher Scientific, USA, Cat# 31460, RRID:AB_228341)/HRPGoat Anti- Mouse secondary antibodies (Thermo Fisher Scientific, USA, Cat# 31430, RRID:AB_228307), AP- Rabbit Anti- Mouse IgG + A + M [H + L] (Thermo Fischer scientific, USA Cat #31329, RRID:AB_228424), AP- Goat Anti- Rabbit IgM (Thermo Fischer scientific, USA, Cat # G- 21079, RRID: AB_2536529), for 1 h at room temperature [27]. .. Proteins were detected using ECL method as well as alkaline phosphatase method; densitometric scanning of protein bands were performed in Bio- Rad Gel Doc XR+ system and analyzed using Image Lab software.



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